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9700 · 10.2

Antibiotics — practice questions

Practice and worked examples for 9700 Antibiotics. Short previews only — attempt the full question in MarkScheme against the official scheme.

Worked example 1

A patient is prescribed a course of antibiotics for a bacterial infection. They stop taking the antibiotics once they feel better, before completing the full course. Explain how this action, over time, can contribute to the development of antibiotic resistance within the general population.

Show solution outline
  1. Initial Infection & Treatment: The patient's bacterial infection initially contains a large population of susceptible bacteria and a very small number of naturally resistant bacteria (present due to random mutation).
  2. Partial Course Effects: Taking the first few doses of antibiotics kills off the most susceptible bacteria, leading to a reduction in symptoms. However, more resilient bacteria, including the resistant mutants, survive.
  3. Selective Pressure and Regrowth: By stopping the course early, the remaining, more tolerant bacteria are left to multiply without competition from the susceptible strains. The antibiotic concentration drops, removing the selective pressure that was keeping them in check.
  4. Reproduction of Resistant Strains: These resistant bacteria reproduce, creating a new population within the patient that has a much higher proportion of resistant individuals. The patient may become ill again or become an asymptomatic carrier of a resistant strain.
  5. Spread of Resistance: The patient can then pass these resistant bacteria to others. If this behaviour is widespread, the frequency of resistant strains in the general population increases, making future infections in other people much harder to treat with that antibiotic.

Worked example 2

A microbiologist performs a Kirby-Bauer disk diffusion test on a culture of E. coli isolated from a patient's urinary tract infection. Disks containing four different antibiotics are placed on an agar plate inoculated with the bacteria. After 24 hours of incubation, the diameters of the zones of inhibition are measured. Using the provided interpretation chart, determine which antibiotic would be the most effective treatment for this infection and explain your reasoning.

Results:

  • Antibiotic A (Penicillin): 0 mm
  • Antibiotic B (Tetracycline): 16 mm
  • Antibiotic C (Ciprofloxacin): 25 mm
  • Antibiotic D (Erythromycin): 14 mm

Interpretation Chart (Zone Diameter in mm):

AntibioticResistant (R)Intermediate (I)Susceptible (S)
Tetracycline≤ 1415-18≥ 19
------------
Ciprofloxacin≤ 1516-20≥ 21
Erythromycin≤ 1314-22≥ 23
Show solution outline
  1. Understand the Principle: The Kirby-Bauer test assesses antibiotic sensitivity. A larger zone of inhibition (the clear area around the disk where bacteria cannot grow) indicates greater effectiveness of the antibiotic against the bacteria.
  2. Step-by-step Analysis:
    • Antibiotic A (Penicillin): The zone of inhibition is 0 mm. This means the bacteria grew right up to the disk, showing no sensitivity. The E. coli strain is completely resistant to Penicillin.
    • Antibiotic B (Tetracycline): The zone is 16 mm. According to the chart, a diameter between 15-18 mm is classified as 'Intermediate' (I). This means the antibiotic has some effect, but it may not be sufficient to effectively treat the infection.
    • Antibiotic C (Ciprofloxacin): The zone is 25 mm. The chart shows that a diameter ≥ 21 mm is classified as 'Susceptible' (S). This indicates that the antibiotic is highly effective at inhibiting the growth of this E. coli strain.
    • Antibiotic D (Erythromycin): The zone is 14 mm. According to the chart, a diameter between 14-22 mm is classified as 'Intermediate' (I). Similar to Tetracycline, its effectiveness is questionable.
  3. Conclusion: The largest zone of inhibition was produced by Ciprofloxacin (25 mm), and this result falls into the 'Susceptible' category. Therefore, Ciprofloxacin is the most effective antibiotic against this specific strain of E. coli and would be the recommended treatment for the patient's infection.