Worked example 1
An experiment was set up to investigate the effect of unilateral light on the growth of an oat coleoptile. The initial length of the coleoptile was 15.0 mm. After 48 hours, the length of the shaded side was measured to be 19.5 mm, and the length of the illuminated side was 16.5 mm. Calculate the percentage elongation for each side and explain the difference.
Show solution outline
- Calculate the elongation of the shaded side:
- Final length - Initial length = 19.5 mm - 15.0 mm = 4.5 mm
- Calculate the percentage elongation of the shaded side:
- (Elongation / Initial length) × 100
- (4.5 mm / 15.0 mm) × 100 = 30%
- Calculate the elongation of the illuminated side:
- Final length - Initial length = 16.5 mm - 15.0 mm = 1.5 mm
- Calculate the percentage elongation of the illuminated side:
- (Elongation / Initial length) × 100
- (1.5 mm / 15.0 mm) × 100 = 10%
- Explanation: The difference in elongation (30% on the shaded side vs. 10% on the illuminated side) is due to the redistribution of auxin. Unilateral light causes auxin to move from the illuminated side to the shaded side. The higher concentration of auxin on the shaded side promotes greater cell elongation, causing it to grow more than the illuminated side. This differential growth results in the coleoptile bending towards the light source.