9700 · 1.1
The microscope in cell studies flashcards
Revision flashcards for Cambridge 9700 The microscope in cell studies (syllabus 1.1). Flip, recall, then mark a real past-paper question.
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What is magnification?
Magnification is the number of times larger an image is compared to the actual size of the object.
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What is resolution?
Resolution is the ability to distinguish between two separate points that are close together. It determines the level of detail that can be seen.
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What is the formula for calculating magnification?
Magnification = Image size / Actual size (M = I / A). Remember to use consistent units for Image and Actual size.
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What limits the resolution of a light microscope?
The resolution of a light microscope is limited by the relatively long wavelength of visible light (around 400-700 nm).
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State a key advantage of a light microscope over an electron microscope.
A light microscope can be used to view living specimens and processes (e.g., cell movement), whereas electron microscopes require dead, fixed specimens.
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What is a major limitation of a Transmission Electron Microscope (TEM)?
The specimen must be extremely thin to allow electrons to pass through, and it only produces a 2D image. The preparation process is also very complex.
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What is a stage micrometer used for?
A stage micrometer is a slide with a precise, known scale used to calibrate the eyepiece graticule at a specific magnification.
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What is the main advantage of a Scanning Electron Microscope (SEM) over a TEM?
An SEM produces a 3D image of the surface of a specimen, providing information about its topography, whereas a TEM provides a 2D cross-sectional view of the internal structure.